Otohime (乙姫) was the de-facto ruler of the Dragon Palace City. Otohime fell in love with an Earth inhabitant called Urashima, when he rescued one of her minions. She is now the owner of Snack Ryuuguujou in Edo, after being rescued by Sakata Gintoki when her palace was being destroyed. She is the main antagonist of Ryugujo Arc.
Once known to be an elegant, refined beauty, she had fallen into despair as she continued to age. She looked very much overweight as the ruler of Ryuuguujou, wearing heavy make-up. After a change of heart, she is a pretty, humble woman (looks to have lost some weight) without the luxurious kimono and make-up.
Prior the storyline of the series, an Earth inhabitant called Urashima was invited to the Dragon Palace City after he rescued one of Otohime's subordinates. He was well treated and spend time with Otohime, who fall in love with him. Urashima told her she was the most beautiful thing he'd ever seen in his life, not only referring to his beauty, also to his kind soul.
Once he returned to Earth, Urashima found out that his village no longer existed, as the time in Earth and in the Palace passed in differently ways. He fall in despair and tried to kill himself, but he was rescued by Otohime by putting him in a cryo-chamber waiting one day he would wake up again.
The soldiers came reported that they caught Kamenashi with some humans. She ordered Kamenashi to be executed and the others to get old. She also wanted them to get the most beautiful one to her. When Otae came, Otohime stated that she isn’t beautiful at all, making Otae go crazy and insult her. She wanted to execute Otae due to her insults. When Otae said to Otohime that she was ugly and didn't know what real beauty was moments before her execution, Otohime dropped her in a pool full of sharks. More humans came to save Tae and freed her from the rope, they teamed up to kill the sharks as well as Otohime's subordinates. After that, Tae beat up the last guards protecting Otohime and finally continued beating Otohime up until she stopped her project. Their fight was interrupted by more subordinates surrounding Tae with their guns, buying Otohime enough time to let the poison spread inside her palace, letting everyone, including her own subordinates, age extremely fast.
She went to stop Gintoki and Katsura, but Hasegawa and Kamenashi tried to stop her. Then Kyubei, and Kagura came and thought that she was their son’s bride and made her lose lots of time. Before they put the antidote to the canon, Otohime caught the antidote and made it fall down. But, the antidote leaked, resulting in Katsura and Gintoki return to their former selves and they easily shot the antidote to the cannon. Everyone in Edo got back to their old selves. Otohime lost and got stuck under the parts of the cannon that was destroyed. The humans saved her. The Ryugu Palce got destroyed and Otohime opened a snack house. Hijikata and Kondo become its regular costumers and Otohime gave advices to the drunk Kondo for every time he got dumped by a woman. Kondo asked to marry with Otohime, but she said that she already has someone, making Hijikata curious. Finally, Urashima came to the shop.